Lettuce variety 79-65 RZ

ABSTRACT

The present invention relates to a  Lactuca sativa  seed designated 79-65 RZ, which exhibits a combination of traits including resistance against downy mildew strains Bl:1 to Bl:31, CA-I to CA-VIII ( Bremia lactucae ), currant-lettuce aphid ( Nasonovia ribisnigri ) biotype Nr:0, and lettuce mosaic virus (LMV), as well as having intensely red, deeply incised, three-dimensional leaves, and a medium to small plant size. The present invention also relates to a  Lactuca sativa  plant produced by growing the 79-65 RZ seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 79-65 RZ.

RELATED APPLICATIONS AND/OR INCORPORATION BY REFERENCE

This application claims priority to and benefit of U.S. provisionalpatent application Ser. No. 61/901,790, filed Nov. 8, 2013.

The foregoing application, and all documents cited therein or duringtheir prosecution (“appin cited documents”) and all documents cited orreferenced in the appin cited documents, and all documents cited orreferenced herein (“herein cited documents”), and all documents cited orreferenced in herein cited documents, together with any manufacturer'sinstructions, descriptions, product specifications, and product sheetsfor any products mentioned herein or in any document incorporated byreference herein, are hereby incorporated herein by reference, and maybe employed in the practice of the invention. More specifically, allreferenced documents are incorporated by reference to the same extent asif each individual document was specifically and individually indicatedto be incorporated by reference.

FIELD OF THE INVENTION

The present invention relates to a new lettuce (Lactuca sativa) varietywhich may exhibit a combination of traits including resistance againstdowny mildew strains Bl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae),currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, and lettucemosaic virus (LMV), as well as having intensely red, deeply incised,three-dimensional leaves, and a medium to small plant size.

BACKGROUND OF THE INVENTION

All cultivated forms of lettuce belong to the highly polymorphicspecies, Lactuca sativa, which is grown for its edible head and leaves.As a crop, lettuces are grown commercially wherever environmentalconditions permit the production of an economically viable yield.

Lactuca sativa is in the Cichoreae tribe of the Asteraceae (Compositae)family. Lettuce is related to chicory, sunflower, aster, scorzonera,dandelion, artichoke and chrysanthemum. Sativa is one of about 300species in the genus Lactuca.

Lettuce cultivars are susceptible to a number of pests and diseases suchas downy mildew (Bremia lactucae). Every year this disease leads tomillions of dollars of lost lettuce crop throughout the world. Downymildew (Bremia lactucae) is highly destructive on lettuce grown atrelatively low temperature and high humidity. Downy mildew is caused bya fungus, Bremia lactucae, which can be one of the following strains:NL1, NL2, NL4, NL5, NL6, NL7, NL10, NL12, NL13, NL14, NL15, NL16, Bl:17,Bl:18, Bl:20, Bl:21, Bl:22, Bl:23, Bl:24, Bl:25, Bl:26, Bl:27, Bl:28,Bl:29, Bl:30, Bl:31 (Van Ettekoven, K. et al., “Identification anddenomination of ‘new’ races of Bremia lactucae,” In: Lebeda, A. andKristkova, E (eds.), Eucarpia Leafy Vegetables, 1999, PalackyUniversity, Olomouc, Czech Republic, pp. 171-175; Van der Arend et al.“Identification and denomination of “new” races of Bremia lactucae inEurope by IBEB until 2002.” In: Van Hintum, Th et al. (eds.), EucarpiaLeafy Vegetables Conference 2003, Centre for Genetic Resources,Wageningen, The Netherlands, p. 151; Plantum NL (Dutch association forbreeding, tissue culture, production and trade of seeds and youngplants), Van der Arend et al. “Identification and denomination of “new”races of Bremia lactucae in Europe by IBEB until 2002.” In: Van Hintum,Th et al. (eds.), Eucarpia Leafy Vegetables Conference 2003, Centre forGenetic Resources, Wageningen, The Netherlands, p. 151; Plantum NL(Dutch association for breeding, tissue culture, production and trade ofseeds and young plants); IBEB press release “New race of Bremia lactucaeBl:27 identified and nominated”, May 2010; Plantum NL (Dutch associationfor breeding, tissue culture, production and trade of seeds and youngplants), “New race of Bremia lactucae Bl:28 identified and nominated”,March 2011; Plantum NL (Dutch association for breeding, tissue culture,production and trade of seeds and young plants), IBEB press release,“New races of Bremia lactucae, Bl:29, Bl:30 and Bl:31 identified andnominated”, August 2013), Ca-I, Ca-IIA, Ca-IIB, Ca-III, Ca-IV(Schettini, T. M., Legg, E. J., Michelmore, R. W., 1991. Insensitivityto metalaxyl in California populations of Bremia lactucae and resistanceof California lettuce cultivars to downy mildew. Phytopathology 81(1).p. 64-70), and Ca-V, Ca-VI, Ca-VII, Ca-VIII (Michelmore R. & Ochoa. O.“Breeding Crisphead Lettuce.” In: California Lettuce Research Board,Annual Report 2005-2006, 2006, Salinas, Calif., pp. 55-68).

Downy mildew causes pale, angular, yellow areas bounded by veins on theupper leaf surfaces. Sporulation occurs on the opposite surface of theleaves. The lesions eventually turn brown, and they may enlarge andcoalesce. These symptoms typically occur first on the lower leaves ofthe lettuce, but under ideal conditions may move into the upper leavesof the head. When the fungus progresses to this degree, the head cannotbe harvested. Less severe damage requires the removal of more leavesthan usual, especially when the lettuce reaches its final destination.

Of the various species of aphids that feed on lettuce, thecurrant-lettuce aphid (Nasonovia ribisnigri) is the most destructivespecies because it feeds both on the leaves of the lettuce as well asthe heart of the lettuce, making it difficult to control withconventional insecticides. The lettuce aphid feeds by sucking sap fromthe lettuce leaves. Although direct damage to the lettuce may belimited, its infestation has serious consequences because the presenceof aphids makes lettuce unacceptable to consumers.

Lettuce mosaic virus (LMV) mainly infects lettuce seeds, which is theprimary way that the virus is introduced to lettuce in the fields, butalso can infect numerous crops and weeds, thereby creating reservoirs ofthe virus. LMV also can be vectored by aphids, which spread the viruswithin a lettuce field and introduce it into lettuce fields frominfected weeds and crops outside the field.

Symptoms of LMV vary greatly. Leaves of plants that are infected at ayoung stage are stunted, deformed and (in some varieties) show a mosaicor mottling pattern. Such plants rarely grow to full size; head lettucevarieties infected early fail to form heads. Plants that are infectedlater in the growth cycle show a different set of symptoms. These plantsmay reach full size, but the older outer leaves turn yellow, twisted,and otherwise are deformed. On head lettuce, the wrapper leaves oftenwill curve back away from the head and developing heads may be deformed.In some cases, brown necrotic flecks occur on the wrapper leaves.

At present the attractive red color in pre-packed lettuce mixtures isoften provided by plant species other than Lactuca sativa. Use is madeof radicchio rosso (Cichorium intybus), red cabbage or red-veinedspinach or red-veined chard. The disadvantage of using leaves from othervegetables than lettuce is that the different taste of these othervegetables is often experienced as undesirable. Radicchio rosso is forexample a Cichorium intybus that has a bitter taste. For taste,red-leaved lettuce (Lactuca sativa) is usually preferred over otherred-leaved species.

The red leaf color of lettuce is caused by anthocyanin in the leaves. Anintensely red colored leaf corresponds with a very high concentration ofanthocyanin in that leaf. Anthocyanin is an antioxidant, whichcontributes to the healthy image of lettuce. Its expression is promotedby UV-light. UV-light is only partly transmitted by glass and plastic.This means that anthocyanin expression of lettuce grown in a glasshouseor plastic tunnel, i.e. indoor production, is less than in the openfield, resulting in less red lettuce plants.

Although several known lettuce cultivars can be harvested mechanicallyat young plant stage, i.e. the babyleaf stage, it is desirable forgrowers and processors to have cultivars that can be harvestedmechanically at mature stage and still provide leaf pieces that are ofsmall, directly edible size and have an attractive deep red color. Thegrower appreciates the yield of a stand of mature plants, which ishigher than the yield of a babyleaf crop. Additionally, mechanicalharvesting saves labor cost and improves labor conditions in comparisonwith commonly applied hand-harvesting methods. The processors appreciateleaves of mature plants because their shelflife is higher than leaves ofbabyleaf plants.

A three-dimensional leaf shape is an important requirement for leavesthat are sold as pre-packaged lettuce which is already cut and washed.It is advantageous to use three-dimensional leaves in pre-packaged bagsbecause such leaves stick less to the plastic film of the bag, making iteasy to fill up the bag. Additionally, since the three-dimensionalshaped leaves do not stick to the plastic film of the bag, it makes itmore attractive for consumers buying pre-packaged lettuce.

The plant size of non-heading lettuce is a measure of yield. This isespecially true in the production of lettuce to be used in processing,where yield is a very important trait. A non-heading lettuce varietyused in processing should preferably have a medium to small plant size.

Citation or identification of any document in this application is not anadmission that such document is available as prior art to the presentinvention.

SUMMARY OF THE INVENTION

There exists a need, therefore, for a lettuce variety which exhibits acombination of traits including resistance against downy mildew strainsBl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae), currant-lettuce aphid(Nasonovia ribisnigri) biotype Nr:0, and lettuce mosaic virus (LMV), aswell as having intensely red, deeply incised, three-dimensional leaves,and a medium to small plant size.

The present invention addresses this need by providing a new type oflettuce (Lactuca sativa) variety, designated 79-65 RZ. Lettuce cultivar79-65 RZ exhibits a combination of traits including resistance againstdowny mildew strains Bl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae),currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, and lettucemosaic virus (LMV), as well as having intensely red, deeply incised,three-dimensional leaves, and a medium to small plant size.

The present invention provides seeds of lettuce cultivar 79-65 RZ, whichhave been deposited with the National Collections of Industrial, Marineand Food Bacteria (NCIMB) in Bucksburn, Aberdeen AB21 9YA, Scotland, UKand have been assigned NCIMB Accession No. 42179.

In one embodiment, the invention provides a lettuce plant designated79-65 RZ, representative seed of which have been deposited under NCIMBAccession No. 42179, wherein said lettuce plant may comprise acombination of traits including resistance against downy mildew strainsBl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae), currant-lettuce aphid(Nasonovia ribisnigri) biotype Nr:0, and lettuce mosaic virus (LMV), aswell as having intensely red, deeply incised, three-dimensional leaves,and a medium to small plant size.

In one embodiment, the invention provides a lettuce plant designated79-65 RZ which may exhibit a combination of traits including resistanceagainst downy mildew strains Bl:1 to Bl:31, CA-I to CA-VIII (Bremialactucae), currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0,and lettuce mosaic virus (LMV), as well as having intensely red, deeplyincised, three-dimensional leaves, a medium to small plant size, andvery slow bolting, representative seed of which have been depositedunder NCIMB Accession No. 42179.

In one embodiment, the invention provides a lettuce plant designated79-65 RZ, representative seed of which have been deposited under NCIMBAccession No. 42179.

In an embodiment of the present invention, there also is provided partsof a lettuce plant of the invention, which may include parts of alettuce plant exhibiting a combination of traits including resistanceagainst downy mildew strains Bl:1 to Bl:31, CA-I to CA-VIII (Bremialactucae), currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0,and lettuce mosaic virus (LMV), as well as having intensely red, deeplyincised, three-dimensional leaves, and a medium to small plant size, orparts of a lettuce plant having any of the aforementioned resistance(s)and a combination of traits including one or more morphological orphysiological characteristics tabulated herein, including parts oflettuce variety 79-65 RZ, wherein the plant parts are involved in sexualreproduction, which include, without limitation, microspores, pollen,ovaries, ovules, embryo sacs or egg cells and/or wherein the plant partsare suitable for vegetative reproduction, which include, withoutlimitation, cuttings, roots, stems, cells or protoplasts and/or whereinthe plant parts are tissue culture of regenerable cells in which thecells or protoplasts of the tissue culture are derived from a tissuesuch as, for example and without limitation, leaves, pollen, embryos,cotyledon, hypocotyls, meristematic cells, roots, root tips, anthers,flowers, seeds or stems. The plants of the invention from which suchparts may come from include those wherein representative seed of whichhas been deposited under NCIMB Accession No. 42179 or lettuce variety orcultivar designated 79-65 RZ, as well as seed from such a plant, plantparts of such a plant (such as those mentioned herein) and plants fromsuch seed and/or progeny of such a plant, advantageously progenyexhibiting such combination of such traits, each of which, is within thescope of the invention; and such combination of traits.

In another embodiment there is a plant grown from seeds, representativeseed of which having been deposited under NCIMB Accession No. 42179.

In a further embodiment there is a plant regenerated from theabove-described plant parts or regenerated from the above-describedtissue culture. Advantageously such a plant may have morphologicaland/or physiological characteristics of lettuce variety 79-65 RZ and/orof a plant grown from seed, representative seed of which having beendeposited under NCIMB Accession No. NCIMB 42179—including withoutlimitation such plants having all of the morphological and physiologicalcharacteristics of lettuce variety 79-65 RZ and/or of plant grown fromseed, representative seed of which having been deposited under NCIMBAccession No. NCIMB 42179. Advantageously, such a plant demonstrates thetraits of resistance against downy mildew strains Bl:1 to Bl:31, CA-I toCA-VIII (Bremia lactucae), currant-lettuce aphid (Nasonovia ribisnigri)biotype Nr:0, and lettuce mosaic virus (LMV), as well as havingintensely red, deeply incised, three-dimensional leaves, and a medium tosmall plant size.

Accordingly, in still a further embodiment, there is provided a lettuceplant having all of the morphological and physiological characteristicsof lettuce variety 79-65 RZ, representative seed of which having beendeposited under NCIMB Accession No. 42179. Such a plant may be grownfrom the seeds, regenerated from the above-described plant parts, orregenerated from the above-described tissue culture. A lettuce planthaving any of the aforementioned resistance(s), and one or moremorphological or physiological characteristics recited or tabulatedherein, and a lettuce plant advantageously having all of theaforementioned resistances and the characteristics recited and tabulatedherein, are preferred. Parts of such plants—such as those plant partsabove-mentioned—are encompassed by the invention.

In one embodiment, there is provided a method for producing a progeny oflettuce cultivar 79-65 RZ which may comprise crossing the a plantdesignated 79-65 RZ with itself or with another lettuce plant,harvesting the resultant seed, and growing said seed.

In a further embodiment there is provided a progeny plant produced bysexual or vegetative reproduction, grown from seeds, regenerated fromthe above-described plant parts, or regenerated from the above-describedtissue culture of the lettuce cultivar or a progeny plant thereof,representative seed of which having been deposited under NCIMB AccessionNo. 42179. The progeny may have any of the aforementioned resistance(s),and one or more morphological or physiological characteristics recitedor tabulated herein, and a progeny plant advantageously having all ofthe aforementioned resistances and the characteristics recited andtabulated herein, are preferred. Advantageously, the progeny demonstratethe traits of resistance against downy mildew strains Bl:1 to Bl:31,CA-I to CA-VIII (Bremia lactucae), currant-lettuce aphid (Nasonoviaribisnigri) biotype Nr:0, and lettuce mosaic virus (LMV), as well ashaving intensely red, deeply incised, three-dimensional leaves, and amedium to small plant size.

Progeny of the lettuce variety 79-65 RZ may be modified in one or moreother characteristics, in which the modification is a result of, forexample and without limitation, mutagenesis or transformation with atransgene.

In still another embodiment, the present invention provides progeny oflettuce cultivar 79-65 RZ produced by sexual or vegetative reproduction,grown from seeds, regenerated from the above-described plant parts, orregenerated from the above-described tissue culture of the lettucecultivar or a progeny plant thereof, in which the regenerated plantshows a combination of traits including resistance against downy mildewstrains Bl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae),currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, and lettucemosaic virus (LMV), as well as having intensely red, deeply incised,three-dimensional leaves, and a medium to small plant size.

In still a further embodiment, the invention provides a method ofproducing a hybrid lettuce seed which may comprise crossing a firstparent lettuce plant with a second parent lettuce plant and harvestingthe resultant hybrid lettuce seed, in which the first parent lettuceplant or the second parent lettuce plant may be a lettuce plant of theinvention, e.g. a lettuce plant having a combination of traits includingresistance against downy mildew strains Bl:1 to Bl:31, CA-I to CA-VIII(Bremia lactucae), currant-lettuce aphid (Nasonovia ribisnigri) biotypeNr:0, and lettuce mosaic virus (LMV), as well as having intensely red,deeply incised, three-dimensional leaves, and a medium to small plantsize and one or more morphological or physiological characteristicstabulated herein, including a lettuce plant of lettuce cultivar 79-65RZ, representative seed of which having been deposited under NCIMB42179.

In another embodiment, the invention provides producing a lettuce plantwhich may exhibit a combination of traits including resistance againstdowny mildew strains Bl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae),currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, and lettucemosaic virus (LMV), as well as having intensely red, deeply incised,three-dimensional leaves, and a medium to small plant size which maycomprise: crossing a mother lettuce plant with a father lettuce plant toproduce a hybrid seed; growing said hybrid seed to produce a hybridplant; selfing said hybrid plant to produce F2 progeny seed; growingsaid F2 progeny seed to produce F2-plants; selecting said F2-plants forexhibiting a combination of traits including resistance against downymildew strains Bl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae),currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, and lettucemosaic virus (LMV), as well as having intensely red, deeply incised,three-dimensional leaves, and a medium to small plant size.

Advantageously the selfing and selection may be repeated; for example atleast once, or at least twice, thrice, four times, five times, six timesor more, to produce F3 or F4 or F5 or F6 or subsequent progeny,especially as progeny from F2 may exhibit the aforementioned combinationof traits, and may be desirable.

In still a further embodiment, the invention provides a method ofproducing a lettuce cultivar which may exhibit a combination of traitsincluding resistance against downy mildew strains Bl:1 to Bl:31, CA-I toCA-VIII (Bremia lactucae), currant-lettuce aphid (Nasonovia ribisnigri)biotype Nr:0, and lettuce mosaic virus (LMV), as well as havingintensely red, deeply incised, three-dimensional leaves, and a medium tosmall plant size.

The invention even further relates to a method of producing lettucewhich may comprise: (a) cultivating to the vegetative plant stage aplant of lettuce variety 79-65 RZ, representative seed of which havingbeen deposited under NCIMB Accession No. NCIMB 42179, and (b) harvestinglettuce leaves or heads from the plant. The invention furthercomprehends packaging and/or processing the lettuce plants, heads orleaves.

Accordingly, it is an object of the invention to not encompass withinthe invention any previously known product, process of making theproduct, or method of using the product such that Applicants reserve theright and hereby disclose a disclaimer of any previously known product,process, or method. It is further noted that the invention does notintend to encompass within the scope of the invention any product,process, or making of the product or method of using the product, whichdoes not meet the written description and enablement requirements of theUSPTO (35 U.S.C. §112, first paragraph) or the EPO (Article 83 of theEPC), such that Applicants reserve the right and hereby disclose adisclaimer of any previously described product, process of making theproduct, or method of using the product.

It is noted that in this disclosure and particularly in the claims,terms such as “comprises”, “comprised”, and “comprising” and the like(e.g., “includes”, “included”, “including”, “contains”, “contained”,“containing”, “has”, “had”, “having”, etc.) can have the meaningascribed to them in US Patent law, i.e., they are open ended terms. Forexample, any method that “comprises,” “has” or “includes” one or moresteps is not limited to possessing only those one or more steps and alsocovers other unlisted steps. Similarly, any plant that “comprises,”“has” or “includes” one or more traits is not limited to possessing onlythose one or more traits and covers other unlisted traits. Similarly,the terms “consists essentially of” and “consisting essentially of” havethe meaning ascribed to them in US Patent law, e.g., they allow forelements not explicitly recited, but exclude elements that are found inthe prior art or that affect a basic or novel characteristic of theinvention. See also MPEP §2111.03. In addition, the term “about” is usedto indicate that a value includes the standard deviation of error forthe device or method being employed to determine the value.

These and other embodiments are disclosed or are obvious from andencompassed by the following Detailed Description.

DEPOSIT

The Deposit with NCIMB Ltd, Ferguson Building, Craibstone Estate,Bucksburn, Aberdeen AB21 9YA, UK, on Oct. 16, 2013, under depositaccession number NCIMB 42179 was made pursuant to the terms of theBudapest Treaty. Upon issuance of a patent, all restrictions upon thedeposit will be removed, and the deposit is intended to meet therequirements of 37 CFR §§1.801-1.809. The deposit will be irrevocablyand without restriction or condition released to the public upon theissuance of a patent. The deposit will be maintained in the depositoryfor a period of 30 years, or 5 years after the last request, or for theeffective life of the patent, whichever is longer, and will be replacedif necessary during that period.

BRIEF DESCRIPTION OF THE DRAWINGS

The following detailed description, given by way of example, but notintended to limit the invention solely to the specific embodimentsdescribed, may best be understood in conjunction with the accompanyingdrawing, in which:

FIG. 1 is an illustration of six different shapes of the fourth leaffrom a 20-day old seedling grown under optimal conditions.

FIG. 2 is a picture of the shape of the fourth leaf of 79-65 RZ.

FIG. 3 is a picture of the shape of a mature leaf of 79-65 RZ.

FIG. 4 is a picture of the shape of the fourth leaf of comparisonvariety Triplex.

FIG. 5 is a picture of the shape of a mature leaf of the comparisonvariety Triplex.

FIG. 6 is a picture that shows the plant size of 79-65 RZ (right) incomparison to plant size of the comparison variety Triplex.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants,seeds and derivatives of a new lettuce variety herein referred to aslettuce variety 79-65 RZ. Lettuce variety 79-65 RZ is a uniform andstable line, distinct from other such lines.

In a preferred embodiment, the specific type of breeding method employedfor developing a lettuce cultivar is pedigree selection, where bothsingle plant selection and mass selection practices are employed.Pedigree selection, also known as the “Vilmorin system of selection,” isdescribed in Fehr, W., Principles of Cultivar Development, Volume I,MacMillan Publishing Co., which is hereby incorporated by reference.

When pedigree selection is applied, in general selection is firstpracticed among F₂ plants. In the next season, the most desirable F₃lines are first identified, and then desirable F₃ plants within eachline are selected. The following season and in all subsequentgenerations of inbreeding, the most desirable families are identifiedfirst, then desirable lines within the selected families are chosen, andfinally desirable plants within selected lines are harvestedindividually. A family refers to lines that were derived from plantsselected from the same progeny from the preceding generation.

Using this pedigree method, two parents may be crossed using anemasculated female and a pollen donor (male) to produce F₁ offspring.Lettuce is an obligate self-pollination species, which means that pollenis shed before stigma emergence, assuring 100% self-fertilization.Therefore, in order to optimize crossing, a method of misting may beused to wash the pollen off prior to fertilization to assure crossing orhybridization.

Parental varieties are selected from commercial varieties thatindividually exhibit one or more desired phenotypes. Additionally, anybreeding method involving selection of plants for the desired phenotypemay be used in the method of the present invention.

The F₁ may be self-pollinated to produce a segregating F₂ generation.Individual plants may then be selected which represent the desiredphenotype in each generation (F₃, F₄, F₅, etc.) until the traits arehomozygous or fixed within a breeding population.

A detailed description of the development of lettuce variety 79-65 RZ isdescribed in Table 1. The seedlot in year 7, seedlot 12R.2717, wasdeposited with the NCIMB under deposit number NCIMB 42179.

TABLE 1 Year Description Location 0 Final F1-cross plants 07B.183948 ×Fijnaart, The Netherlands 07P.36224 in glass house. 1 F1 plant grown forF2 seed production. Fijnaart, The Netherlands 2 F2 plant selected inopen field, Fijnaart, The Netherlands followed by F3 seed production. 3F3 plant selected in open field, Fijnaart, The Netherlands followed byF4 seed production. 4 F4 plant selected in plastic tunnel, Daylesford,Australia followed by F5 seed production. 5 F5 plant selected in openfield, Fijnaart, The Netherlands followed by F6 seed production. 6 F6plant selected in glass house, Hoek van Holland, The followed by F7 seedproduction. Netherlands 7 F7 line established uniform, multiplied Hoekvan Holland, The in glass house: seedlot 12R.2717. Netherlands

In one embodiment, a plant of the invention has all the morphologicaland physiological characteristics of lettuce variety 79-65 RZ. Thesecharacteristics of a lettuce plant of the invention, e.g. variety 79-65RZ, are summarized in Tables 2, 3, and 4.

Next to the morphological and physiological characteristics mentioned inTables 2, 3, and 4, a plant of the invention also exhibits resistance todowny mildew (Bremia lactucae Regel.).

As used herein resistance against Bremia lactucae is defined as theability of a plant to resist infection by each of the various strainsBl:1 to Bl:31, Ca-I to Ca-VIII of Bremia lactucae Regel. in all stagesbetween the seedling stage and the harvestable plant stage. Bl:1 toBl:31 means strains NL1, NL2, NL4, NL5, NL6, NL7, NL10, NL12, NL13,NL14, NL15, NL16, Bl:17, Bl:18, Bl:20, Bl:21, Bl:22, Bl:23, Bl:24,Bl:25, Bl:26, Bl:27, Bl:28, Bl:29, Bl:30, Bl:31 (Van Ettekoven K, Vander Arend A J M, 1999. identification and denomination of ‘new’ races ofBremia lactucae. In: Lebeda A, Kristkova E (eds.) Eucarpia leafyvegetables '99. Palacky University, Olomouc, Czech Republic, 1999:171-175; Van der Arend, A. J. M., Gautier, J., Guenard, M., Michel, H.,Moreau, B., de Ruijter, J., Schut, J. W. and de Witte, I. (2003).Identification and denomination of ‘new’ races of Bremia lactucae inEurope by IBEB until 2002. In: Eucarpia leafy vegetables 2003.Proceedings of the Eucarpia Meeting on leafy vegetables genetics andbreeding. Noorwijkerhout, The Netherlands. Eds. Van Hintum T., LebedaA., Pink D., Schut J. pp 151-160; Van der Arend A J M, Gautier J,Grimault V, Kraan P, Van der Laan R, Mazet J, Michel H, Schut J W,Smilde D, De Witte I (2006) Identification and denomination of “new”races of Bremia lactucae in Europe by IBEB until 2006; incorporatedherein by reference; Plantum NL (Dutch association for breeding, tissueculture, production and trade of seeds and young plants), IBEB pressrelease, “New race of Bremia lactucae Bl:27 identified and nominated”,May 2010; Plantum NL (Dutch association for breeding, tissue culture,production and trade of seeds and young plants), IBEB press release,“New race of Bremia lactucae Bl:28 identified and nominated”, March2011; Plantum NL (Dutch association for breeding, tissue culture,production and trade of seeds and young plants), IBEB press release,“New races of Bremia lactucae, Bl:29, Bl:30 and Bl:31 identified andnominated”, August 2013). Ca-I to Ca-VIII means Ca-I, Ca-IIA, Ca-IIB,Ca-III, Ca-IV (Schettini, T. M., Legg, E. J., Michelmore, R. W., 1991.Insensitivity to metalaxyl in California populations of Bremia lactucaeand resistance of California lettuce cultivars to downy mildew,Phytopathology 81(1). p. 64-70), and Ca-V, Ca-VI, Ca-VII, Ca-VIII(Michelmore R. & Ochoa. O. “Breeding Crisphead Lettuce.” In: CaliforniaLettuce Research Board, Annual Report 2005-2006, 2006, Salinas, Calif.,pp. 55-68).

Resistance typically is tested by two interchangeable methods, describedby Bonnier, F. J. M. et al. (Euphytica, 61(3):203-211, 1992;incorporated herein by reference). One method involves inoculating 7-dayold seedlings, and observing sporulation 10 to 14 days later. The othermethod involves inoculating leaf discs with a diameter of 18 mm obtainedfrom a non-senescent, fully grown true leaf and observing sporulation 10days later.

As used herein, resistance against Nasonovia ribisnigri (Mosley), orcurrant-lettuce aphid, is defined as the plant characteristic whichresults in a non-feeding response of the aphid on the leaves of theplant in all stages between 5 true-leaf stage and harvestable plantstage (U.S. Pat. No. 5,977,443 to Jansen, J. P. A., “Aphid Resistance inComposites,” p. 12, 1999; incorporated herein by reference).

Resistance is tested by spreading at least ten aphids of biotype Nr:0 ona plant in a plant stage between 5 true leaves and harvestable stage,and observing the density of the aphid population on the plant as wellas the growth reduction after 14 days in a greenhouse, with temperaturesettings of 23 degrees Celsius in daytime and 21 degrees Celsius atnight. Day length is kept at 18 hours by assimilation lights.

As used herein, resistance against lettuce mosaic virus (LMV) may bedefined as the ability of the plant to grow normally after LMV infectionand to inhibit the virus transmission to seed. Resistance may be testedby mechanical inoculation of young plants in a climate cell orgreenhouse, as described by Pink, D. A. C. et al. (Plant Pathology,41(1): 5-12, 1992), incorporated herein by reference. Inoculatedresistant plants grow just as well as uninoculated plants and show nochlorosis or mosaic symptoms. The LMV isolate, which may be used fortesting, is Ls-1 (International Union for the Protection of NewVarieties of Plants [UPOV], Guidelines for the conduct of tests fordistinctness, uniformity and stability; lettuce (Lactuca sativa L.),2002, p. 35; incorporated herein by reference).

As used herein, intensely red leaves are defined as mature leaves withan anthocyanin distribution throughout the leaf, and an anthocyaninconcentration that is comparable to or stronger than the varietyTriplex. The mature leaves of 79-65 RZ have an anthocyanin distributionthroughout, and an anthocyanin concentration that is intense to veryintense.

As used herein, the characteristics of the leaf blade is by comparisonto the standard variety Lagon (UPOV guidelines for the conduct of testsfor distinctness, uniformity and stability, TG/13/10, UPOV, Geneva,2006). 79-65 RZ has deeply incised leaves, which is defined as leaveswhich are divided, flabellate and have deep incisions on the margin ofthe apical part.

As used herein, three-dimensional leaves are characterized as matureleaves having a combination of deep leaf incisions, moderate to strongundulations of the apical leaf margin, which is shallowly dentate todeeply dentate. 79-65 RZ has three-dimensional leaves.

As used herein, moderate undulations of the apical leaf margin, is adegree of undulation that is comparable to or stronger than the varietyTriplex grown under long-day outdoor conditions at a latitude of 52°,i.e. sowing 100 days before the longest day, in an Oceanic climate(Köppen-classification: Cfb; McKnight & Hess, 2000. Physical Geography:A Landscape Appreciation. Upper Saddle River, N.J.: Prentice Hall).

As used herein, a shallowly dentate to deeply dentate margin of a matureleaf, is a leaf margin which has a comparable or stronger indentationthan the variety Triplex grown under long-day outdoor conditions at alatitude of 52°, i.e. sowing 100 days before the longest day, in anOceanic climate (Köppen-classification: Cfb; McKnight & Hess, 2000.Physical Geography: A Landscape Appreciation. Upper Saddle River, N.J.:Prentice Hall).

As used herein, a medium to small plant size is a plant size, which iscomparable to the variety Wintex grown under long-day outdoor conditionsat a latitude of 52°, i.e. sowing 100 days before the longest day, in anOceanic climate (Koppen-classification: Cfb; McKnight & Hess, 2000.Physical Geography: A Landscape Appreciation. Upper Saddle River, N.J.:Prentice Hall).

As used herein, very slow bolting is bolting speed, which is comparableto or slower than the variety Triplex grown under long-day outdoorconditions at a latitude of 52°, i.e. sowing 100 days before the longestday, in an Oceanic climate (Koppen-classification: Cfb; McKnight & Hess,2000. Physical Geography: A Landscape Appreciation. Upper Saddle River,N.J.: Prentice Hall).

Embodiments of the inventions advantageously have one or more, and mostadvantageously all, of these characteristics.

In Table 2, the seed color, cotyledon shape and characteristics of thefourth leaf of 79-65 RZ is compared with Triplex (79-69 RZ) and RedSalad Bowl.

TABLE 2 Red Character 79-65 RZ Triplex Salad Bowl Plant TypeCutting/leaf Cutting/leaf Cutting/leaf Seed Color Black Black BlackCotyledon Shape Intermediate Intermediate Intermediate Shape of FourthLeaf Pinnately lobed Pinnately Pinnately to incised lobed to lobedincised Rolling of Fourth Leaf Present Present Present Cupping of FourthLeaf Uncupped Uncupped Uncupped Fourth Leaf Apical Margin IncisedIncised Lobed Fourth Leaf Basal Margin Incised Incised Lobed UndulationMedium Medium Slight to slight Green color Red Red Red Anthocyanindistribution Throughout Throughout Throughout Anthocyanin concentrationIntense Intense Moderate

In Table 3, the mature leaf and head characteristics of 79-65 RZ iscompared with Triplex RZ (79-69 RZ) and Red Salad Bowl. RHS=RoyalHorticulural Society colour chart code.

TABLE 3 Red Character 79-65 RZ Triplex Salad Bowl Leaf Color Red Red RedAnthocyanin Throughout Throughout Throughout Distribution AnthocyaninIntense to very Intense Moderate concentration intense Margin IncisionDeep to Deep Moderate Depth moderate Leaf Size Medium Medium to smallMedium Leaf Glossiness Glossy Glossy to moderate Moderate LeafBlistering Absent Absent Absent Trichomes Absent Absent Absent HeadShape Non-heading Non-heading Non-heading Plant size Medium to Small tomedium Medium to small large Butt Shape Rounded Rounded Rounded to flatMidrib Prominently Moderately raised Moderately raised raised Coreheight (mm) 19 32 47 from base of head to apex Bolting class Very slowVery slow Slow

In Table 4, the main characteristics of 79-65 RZ are compared with“Duplex”, “Triplex”, “Wintex”, “Telex”, and “Stefano”.

TABLE 4 Duplex (79- Triplex Wintex (79- Telex (79- Stefano (79-Character 79-65 RZ 02 RZ) (79-69 RZ) 86 RZ) 51 RZ) 93 RZ) resistance toBl:1 to Bl:31 Bl:1 to Bl:1 to Bl:30 Bl:1 to Bl:30 Bl:1 to Bl:31 Bl:1 toBremia & CA-I to Bl:28, Bl:30, & CA-I to & CA-I to & CA-I to Bl:31 &lactucae CA-VIII Bl:31 & CA- CA-VIII CA-VIII CA-VIII CA-I to CA- race: Ito CA-VIII VIII resistance to Resistant Resistant Resistant ResistantResistant Resistant Nasonovia ribisnigri biotype Nr:0 resistance toResistant Susceptible Susceptible Susceptible Resistant Susceptible LMVMature leaf: Intensely red Medium red Intensely red Intensely red Mediumred Intensely red red coloration mature leaf: Moderate to ModerateModerate Moderate Moderate to Slight degree of strong strong undulationof apical leaf margin mature leaf: Shallowly Shallowly ShallowlyShallowly Shallowly Shallowly indentation dentate to dentate to dentateto dentate to dentate to dentate of leaf deeply deeply deeply deeplydeeply margin dentate denate denate denate denate

In an embodiment, the invention relates to lettuce plants that has allthe morphological and physiological characteristics of the invention andhave acquired said characteristics by introduction of the geneticinformation that is responsible for the characteristics from a suitablesource, either by conventional breeding, or genetic modification, inparticular by cisgenesis or transgenesis. Cisgenesis is geneticmodification of plants with a natural gene, coding for an (agricultural)trait, from the crop plant itself or from a sexually compatible donorplant. Transgenesis is genetic modification of a plant with a gene froma non-crossable species or a synthetic gene.

Just as useful traits that may be introduced by backcrossing, usefultraits may be introduced directly into the plant of the invention, beinga plant of lettuce variety 79-65 RZ, by genetic transformationtechniques; and, such plants of lettuce variety 79-65 RZ that haveadditional genetic information introduced into the genome or thatexpress additional traits by having the DNA coding there for introducedinto the genome via transformation techniques, are within the ambit ofthe invention, as well as uses of such plants, and the making of suchplants.

Genetic transformation may therefore be used to insert a selectedtransgene into the plant of the invention, being a plant of lettucevariety 79-65 RZ or may, alternatively, be used for the preparation oftransgenes which may be introduced by backcrossing. Methods for thetransformation of plants, including lettuce, are well known to those ofskill in the art.

Vectors used for the transformation of lettuce cells are not limited solong as the vector may express an inserted DNA in the cells. Forexample, vectors which may comprise promoters for constitutive geneexpression in lettuce cells (e.g., cauliflower mosaic virus 35Spromoter) and promoters inducible by exogenous stimuli may be used.Examples of suitable vectors include pBI binary vector. The “lettucecell” into which the vector is to be introduced includes various formsof lettuce cells, such as cultured cell suspensions, protoplasts, leafsections, and callus. A vector may be introduced into lettuce cells byknown methods, such as the polyethylene glycol method, polycationmethod, electroporation, Agrobacterium-mediated transfer, particlebombardment and direct DNA uptake by protoplasts. To effecttransformation by electroporation, one may employ either friabletissues, such as a suspension culture of cells or embryogenic callus oralternatively one may transform immature embryos or other organizedtissue directly. In this technique, one would partially degrade the cellwalls of the chosen cells by exposing them to pectin-degrading enzymes(pectolyases) or mechanically wound tissues in a controlled manner.

A particularly efficient method for delivering transforming DNA segmentsto plant cells is microprojectile bombardment. In this method, particlesare coated with nucleic acids and delivered into cells by a propellingforce. Exemplary particles include those which may be comprised oftungsten, platinum, and preferably, gold. For the bombardment, cells insuspension are concentrated on filters or solid culture medium.Alternatively, immature embryos or other target cells may be arranged onsolid culture medium. The cells to be bombarded are positioned at anappropriate distance below the macroprojectile stopping plate. Anillustrative embodiment of a method for delivering DNA into plant cellsby acceleration is the Biolistics Particle Delivery System, which may beused to propel particles coated with DNA or cells through a screen, suchas a stainless steel or Nytex screen, onto a surface covered with targetlettuce cells. The screen disperses the particles so that they are notdelivered to the recipient cells in large aggregates. It is believedthat a screen intervening between the projectile apparatus and the cellsto be bombarded reduces the size of projectiles aggregate and maycontribute to a higher frequency of transformation by reducing thedamage inflicted on the recipient cells by projectiles that are toolarge. Microprojectile bombardment techniques are widely applicable, andmay be used to transform virtually any plant species, including a plantof lettuce variety 79-65 RZ.

Agrobacterium-mediated transfer is another widely applicable system forintroducing gene loci into plant cells. An advantage of the technique isthat DNA may be introduced into whole plant tissues, thereby bypassingthe need for regeneration of an intact plant from a protoplast.Agrobacterium transformation vectors are capable of replication in E.coli as well as Agrobacterium, allowing for convenient manipulations.Moreover, advances in vectors for Agrobacterium-mediated gene transferhave improved the arrangement of genes and restriction sites in thevectors to facilitate the construction of vectors capable of expressingvarious polypeptide coding genes. The vectors have convenientmulti-linker regions flanked by a promoter and a polyadenylation sitefor direct expression of inserted polypeptide coding genes.Additionally, Agrobacterium containing both armed and disarmed Ti genesmay be used for transformation. In those plant strains whereAgrobacterium-mediated transformation is efficient, it is the method ofchoice because of the facile and defined nature of the gene locustransfer. The use of Agrobacterium-mediated plant integrating vectors tointroduce DNA into plant cells, including lettuce plant cells, is wellknown in the art (See, e.g., U.S. Pat. Nos. 7,250,560 and 5,563,055).

Transformation of plant protoplasts also may be achieved using methodsbased on calcium phosphate precipitation, polyethylene glycol treatment,electroporation, and combinations of these treatments.

A number of promoters have utility for plant gene expression for anygene of interest including but not limited to selectable markers,scoreable markers, genes for pest tolerance, disease resistance,nutritional enhancements and any other gene of agronomic interest.Examples of constitutive promoters useful for lettuce plant geneexpression include, but are not limited to, the cauliflower mosaic virus(CaMV) P-35S promoter, a tandemly duplicated version of the CaMV 35Spromoter, the enhanced 35S promoter (P-e35S), the nopaline synthasepromoter, the octopine synthase promoter, the figwort mosaic virus(P-FMV) promoter (see U.S. Pat. No. 5,378,619), an enhanced version ofthe FMV promoter (P-eFMV) where the promoter sequence of P-FMV isduplicated in tandem, the cauliflower mosaic virus 19S promoter, asugarcane bacilliform virus promoter, a commelina yellow mottle viruspromoter, the promoter for the thylakoid membrane proteins from lettuce(psaD, psaF, psaE, PC, FNR, atpC, atpD, cab, rbcS) (see U.S. Pat. No.7,161,061), the CAB-1 promoter from lettuce (see U.S. Pat. No.7,663,027), the promoter from maize prolamin seed storage protein (seeU.S. Pat. No. 7,119,255), and other plant DNA virus promoters known toexpress in plant cells. A variety of plant gene promoters that areregulated in response to environmental, hormonal, chemical, and/ordevelopmental signals may be used for expression of an operably linkedgene in plant cells, including promoters regulated by (1) heat, (2)light (e.g., pea rbcS-3A promoter, maize rbcS promoter, or chlorophylla/b-binding protein promoter), (3) hormones, such as abscisic acid, (4)wounding (e.g., wunl, or (5) chemicals such as methyl jasmonate,salicylic acid, or Safener. It may also be advantageous to employorgan-specific promoters.

Exemplary nucleic acids which may be introduced to the lettuce varietyof this invention include, for example, DNA sequences or genes fromanother species, or even genes or sequences which originate with or arepresent in lettuce species, but are incorporated into recipient cells bygenetic engineering methods rather than classical reproduction orbreeding techniques. However, the term “exogenous” is also intended torefer to genes that are not normally present in the cell beingtransformed, or perhaps simply not present in the form, structure, etc.,as found in the transforming DNA segment or gene, or genes which arenormally present and that one desires to express in a manner thatdiffers from the natural expression pattern, e.g., to over-express.Thus, the term “exogenous” gene or DNA is intended to refer to any geneor DNA segment that is introduced into a recipient cell, regardless ofwhether a similar gene may already be present in such a cell. The typeof DNA included in the exogenous DNA may include DNA which is alreadypresent in the plant cell, DNA from another plant, DNA from a differentorganism, or a DNA generated externally, such as a DNA sequencecontaining an antisense message of a gene, or a DNA sequence encoding asynthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and couldpotentially be introduced into a plant of lettuce variety 79-65 RZ.Non-limiting examples of particular genes and corresponding phenotypesone may choose to introduce into a lettuce plant include one or moregenes for insect tolerance, pest tolerance such as genes for fungaldisease control, herbicide tolerance, and genes for quality improvementssuch as yield, nutritional enhancements, environmental or stresstolerances, or any desirable changes in plant physiology, growth,development, morphology or plant product(s).

Alternatively, the DNA coding sequences may affect these phenotypes byencoding a non-translatable RNA molecule that causes the targetedinhibition of expression of an endogenous gene, for example viaantisense- or cosuppression-mediated mechanisms. The RNA could also be acatalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desiredendogenous mRNA product. Thus, any gene which produces a protein or mRNAwhich expresses a phenotype or morphology change of interest is usefulfor the practice of the present invention. (See also U.S. Pat. No.7,576,262, “Modified gene-silencing RNA and uses thereof.”)

U.S. Pat. Nos. 7,230,158, 7,122,720, 7,081,363, 6,734,341, 6,503,732,6,392,121, 6,087,560, 5,981,181, 5,977,060, 5,608,146, 5,516,667, eachof which, and all documents cited therein are hereby incorporated hereinby reference, consistent with the above INCORPORATION BY REFERENCEsection, are additionally cited as examples of U.S. patents that mayconcern transformed lettuce and/or methods of transforming lettuce orlettuce plant cells, and techniques from these US patents, as well aspromoters, vectors, etc., may be employed in the practice of thisinvention to introduce exogenous nucleic acid sequence(s) into a plantof lettuce variety 79-65 RZ (or cells thereof), and exemplify someexogenous nucleic acid sequence(s) which may be introduced into a plantof lettuce variety 79-65 RZ (or cells thereof) of the invention, as wellas techniques, promoters, vectors etc., to thereby obtain further plantsof lettuce variety 79-65 RZ, plant parts and cells, seeds, otherpropagation material harvestable parts of these plants, etc. of theinvention, e.g. tissue culture, including a cell or protoplast, such asan embryo, meristem, cotyledon, pollen, leaf, anther, root, root tip,pistil, flower, seed or stalk.

The invention further relates to propagation material for producingplants of the invention. Such propagation material may comprise interalia seeds of the claimed plant and parts of the plant that are involvedin sexual reproduction. Such parts are for example selected from thegroup consisting of seeds, microspores, pollen, ovaries, ovules, embryosacs and egg cells. In addition, the invention relates to propagationmaterial which may comprise parts of the plant that are suitable forvegetative reproduction, for example cuttings, roots, stems, cells,protoplasts.

According to a further aspect thereof the propagation material of theinvention may comprise a tissue culture of the claimed plant. The tissueculture may comprise regenerable cells. Such tissue culture may bederived from leaves, pollen, embryos, cotyledon, hypocotyls,meristematic cells, roots, root tips, anthers, flowers, seeds and stems.(See generally U.S. Pat. No. 7,041,876 on lettuce being recognized as aplant that may be regenerated from cultured cells or tissue).

Also, the invention comprehends methods for producing a seed of a 79-65RZ-derived lettuce plant which may comprise (a) crossing a plant oflettuce variety 79-65 RZ, representative seed of which having beendeposited under NCIMB Accession No. NCIMB 42179, with a second lettuceplant, and (b) whereby seed of a 79-65 RZ-derived lettuce plant forms.Such a method may further comprise (c) crossing a plant grown from 79-65RZ-derived lettuce seed with itself or with a second lettuce plant toyield additional 79-65 RZ-derived lettuce seed, (d) growing theadditional 79-65 RZ-derived lettuce seed of step (c) to yield additional79-65 RZ-derived lettuce plants, and (e) repeating the crossing andgrowing of steps (c) and (d) for an additional 3-10 generations tofurther generate 79-65 RZ-derived lettuce plants.

The invention further relates to the above methods that may furthercomprise selecting at steps b), d), and e), a 79-65 RZ-derived lettuceplant, exhibiting a combination of traits including resistance againstdowny mildew strains Bl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae),currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, and lettucemosaic virus (LMV), as well as having intensely red, deeply incised,three-dimensional leaves, and a medium to small plant size.

In particular, the invention relates to methods for producing a seed ofa 79-65 RZ-derived lettuce plant which may comprise (a) crossing a plantof lettuce variety 79-65 RZ, representative seed of which having beendeposited under NCIMB Accession No. NCIMB 42179, with a second lettuceplant and (b) whereby seed of a 79-65 RZ-derived lettuce plant forms,wherein such a method may further comprise (c) crossing a plant grownfrom 79-65 RZ-derived lettuce seed with itself or with a second lettuceplant to yield additional 79-65 RZ-derived lettuce seed, (d) growing theadditional 79-65 RZ-derived lettuce seed of step (c) to yield additional79-65 RZ-derived lettuce plants and selecting plants exhibiting acombination of the traits including resistance against downy mildewstrains Bl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae),currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, and lettucemosaic virus (LMV), as well as having intensely red, deeply incised,three-dimensional leaves, and a medium to small plant size, and (e)repeating the crossing and growing of steps (c) and (d) for anadditional 3-10 generations to further generate 79-65 RZ-derived lettuceplants that exhibit a combination of traits including resistance againstdowny mildew strains Bl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae),currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, and lettucemosaic virus (LMV), as well as having intensely red, deeply incised,three-dimensional leaves, and a medium to small plant size.

The invention additionally provides a method of introducing a desiredtrait into a plant of lettuce variety 79-65 RZ which may comprise: (a)crossing a plant of lettuce variety 79-65 RZ, representative seed ofwhich having been deposited under NCIMB Accession No. NCIMB 42179, witha second lettuce plant that may comprise a desired trait to produce F1progeny; (b) selecting an F1 progeny that may comprise the desiredtrait; (c) crossing the selected F1 progeny with a plant of lettucevariety 79-65 RZ, to produce backcross progeny; (d) selecting backcrossprogeny which may comprise the desired trait and the physiological andmorphological characteristic of a plant of lettuce variety 79-65 RZ;and, optionally, (e) repeating steps (c) and (d) one or more times insuccession to produce selected fourth or higher backcross progeny thatmay comprise the desired trait and all of the physiological andmorphological characteristics of a plant of lettuce variety 79-65 RZ,when grown in the same environmental conditions. The invention, ofcourse, includes a lettuce plant produced by this method.

Backcrossing may also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or non-inbredsource to an inbred that lacks that trait. This may be accomplished, forexample, by first crossing a superior inbred (A) (recurrent parent) to adonor inbred (non-recurrent parent), which carries the appropriate locusor loci for the trait in question. The progeny of this cross are thenmated back to the superior recurrent parent (A) followed by selection inthe resultant progeny for the desired trait to be transferred from thenon-recurrent parent. After five or more backcross generations withselection for the desired trait, the progeny are heterozygous for locicontrolling the characteristic being transferred, but are like thesuperior parent for most or almost all other loci. The last backcrossgeneration would be selfed to give pure breeding progeny for the traitbeing transferred. When a plant of lettuce variety 79-65 RZ,representative seed of which having been deposited under NCIMB AccessionNo. NCIMB 42179, is used in backcrossing, offspring retaining thecombination of traits including resistance against downy mildew strainsBl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae), currant-lettuce aphid(Nasonovia ribisnigri) biotype Nr:0, and lettuce mosaic virus (LMV), aswell as having intensely red, deeply incised, three-dimensional leaves,and a medium to small plant size are progeny within the ambit of theinvention. Backcrossing methods may be used with the present inventionto improve or introduce a characteristic into a plant of the invention,being a plant of lettuce variety 79-65 RZ. See, e.g., U.S. Pat. No.7,705,206 (incorporated herein by reference consistent with the aboveINCORPORATION BY REFERENCE section), for a general discussion relatingto backcrossing.

The invention further involves a method of determining the genotype of aplant of lettuce variety 79-65 RZ, representative seed of which has beendeposited under NCIMB Accession No. NCIMB 42179, or a first generationprogeny thereof, which may comprise obtaining a sample of nucleic acidsfrom said plant and detecting in said nucleic acids a plurality ofpolymorphisms. This method may additionally comprise the step of storingthe results of detecting the plurality of polymorphisms on a computerreadable medium. The plurality of polymorphisms are indicative of and/orgive rise to the expression of the morphological and physiologicalcharacteristics of lettuce variety 79-65 RZ.

There are various ways of obtaining genotype data from a nucleic acidsample. Genotype data may be gathered which is specific for certainphenotypic traits (e.g. gene sequences), but also patterns of randomgenetic variation may be obtained to construct a so-called DNAfingerprint. Depending on the technique used a fingerprint may beobtained that is unique for lettuce variety 79-65 RZ. Obtaining a uniqueDNA fingerprint depends on the genetic variation present in a varietyand the sensitivity of the fingerprinting technique. A technique knownin the art to provide a good fingerprint profile is called AFLPfingerprinting technique (See generally U.S. Pat. No. 5,874,215), butthere are many other marker based techniques, such as RFLP (orRestriction fragment length polymorphism), SSLP (or Simple sequencelength polymorphism), RAPD (or Random amplification of polymorphic DNA)VNTR (or Variable number tandem repeat), Microsatellite polymorphism,SSR (or Simple sequence repeat), STR (or Short tandem repeat), SFP (orSingle feature polymorphism) DArT (or Diversity Arrays Technology), RADmarkers (or Restriction site associated DNA markers) (e.g. Baird et al.PloS One Vol. 3 e3376, 2008; Semagn et al. African Journal ofBiotechnology Vol. 5 number 25 pp. 2540-2568, 29 Dec., 2006). Nowadays,sequence-based methods are utilizing Single Nucleotide Polymorphisms(SNPs) that are randomly distributed across genomes, as a common toolfor genotyping (e.g. Elshire et al. PloS One Vol. 6: e19379, 2011;Poland et al. PloS One Vol. 7: e32253; Truong et al. PLoS One Vol. 7number 5: e37565, 2012).

With any of the aforementioned genotyping techniques, polymorphisms maybe detected when the genotype and/or sequence of the plant of interestis compared to the genotype and/or sequence of one or more referenceplants. As used herein, the genotype and/or sequence of a referenceplant may be derived from, but is not limited to, any one of thefollowing: parental lines, closely related plant varieties or species,complete genome sequence of a related plant variety or species, or thede novo assembled genome sequence of one or more related plant varietiesor species. For example, it is possible to detect polymorphisms for thecharacteristic of Bremia resistance and/or LMV resistance by comparingthe genotype and/or the sequence of lettuce variety 79-65 RZ with thegenotype and/or the sequence of one or more reference plants. Thereference plant(s) used for comparison may for example be, but is notlimited to, any of the comparison varieties Duplex, Triplex, Wintex,Telex, or Stefano.

The polymorphism revealed by these techniques may be used to establishlinks between genotype and phenotype. The polymorphisms may thus be usedto predict or identify certain phenotypic characteristics, individuals,or even species. The polymorphisms are generally called markers. It iscommon practice for the skilled artisan to apply molecular DNAtechniques for generating polymorphisms and creating markers.

The polymorphisms of this invention may be provided in a variety ofmediums to facilitate use, e.g. a database or computer readable medium,which may also contain descriptive annotations in a form that allows askilled artisan to examine or query the polymorphisms and obtain usefulinformation.

As used herein “database” refers to any representation of retrievablecollected data including computer files such as text files, databasefiles, spreadsheet files and image files, printed tabulations andgraphical representations and combinations of digital and image datacollections. In a preferred aspect of the invention, “database” refersto a memory system that may store computer searchable information.

As used herein, “computer readable media” refers to any medium that maybe read and accessed directly by a computer. Such media include, but arenot limited to: magnetic storage media, such as floppy discs, hard disc,storage medium and magnetic tape; optical storage media such as CD-ROM;electrical storage media such as RAM, DRAM, SRAM, SDRAM, ROM; and PROMs(EPROM, EEPROM, Flash EPROM), and hybrids of these categories such asmagnetic/optical storage media. A skilled artisan may readily appreciatehow any of the presently known computer readable mediums may be used tocreate a manufacture which may comprise computer readable medium havingrecorded thereon a polymorphism of the present invention.

As used herein, “recorded” refers to the result of a process for storinginformation in a retrievable database or computer readable medium. Forinstance, a skilled artisan may readily adopt any of the presently knownmethods for recording information on computer readable medium togenerate media which may comprise the polymorphisms of the presentinvention. A variety of data storage structures are available to askilled artisan for creating a computer readable medium where the choiceof the data storage structure will generally be based on the meanschosen to access the stored information. In addition, a variety of dataprocessor programs and formats may be used to store the polymorphisms ofthe present invention on computer readable medium.

The present invention further provides systems, particularlycomputer-based systems, which contain the polymorphisms describedherein. Such systems are designed to identify the polymorphisms of thisinvention. As used herein, “a computer-based system” refers to thehardware, software and memory used to analyze the polymorphisms. Askilled artisan may readily appreciate that any one of the currentlyavailable computer-based system are suitable for use in the presentinvention.

Lettuce leaves are sold in packaged form, including without limitationas pre-packaged lettuce salad or as lettuce heads. Mention is made ofU.S. Pat. No. 5,523,136, incorporated herein by reference consistentwith the above INCORPORATION BY REFERENCE section, which providespackaging film, and packages from such packaging film, including suchpackaging containing leafy produce, and methods for making and usingsuch packaging film and packages, which are suitable for use with thelettuce leaves of the invention. Thus, the invention comprehends the useof and methods for making and using the leaves of the lettuce plant ofthe invention, as well as leaves of lettuce plants derived from theinvention. The invention further relates to a container which maycomprise one or more plants of the invention, or one or more lettuceplants derived from a plant of the invention, in a growth substrate forharvest of leaves from the plant in a domestic environment. This way theconsumer may pick very fresh leaves for use in salads. More generally,the invention includes one or more plants of the invention or one ormore plants derived from lettuce of the invention, wherein the plant isin a ready-to-harvest condition, including with the consumer picking hisown, and further including a container which may comprise one or more ofthese plants.

The invention is further described by the following numbered paragraphs:

1. A lettuce plant designated 79-65 RZ, representative seed of whichhaving been deposited under NCIMB Accession No. 42179, wherein saidplant comprises at least the following combination of traits includingresistance against downy mildew strains Bl:1 to Bl:31, CA-I to CA-VIII(Bremia lactucae), currant-lettuce aphid (Nasonovia ribisnigri) biotypeNr:0, and lettuce mosaic virus (LMV), as well as having intensely red,deeply incised, three-dimensional leaves, and a medium to small plantsize.

2. The lettuce plant of paragraph 1 wherein the plant is very slowbolting.

3. A seed of the plant of paragraph 1.

4. Parts of the plant of paragraph 1, wherein said parts of the plantare suitable for sexual reproduction.

5. Parts of the plant of paragraph 4, wherein said parts are selectedfrom the group consisting of microspores, pollen, ovaries, ovules,embryo sacs and egg cells.

6. Parts of the plant of paragraph 1, wherein said parts of the plantare suitable for vegetative reproduction.

7. Parts of paragraph 6, wherein said parts are selected from the groupconsisting of cuttings, roots, stems, cells and protoplasts.

8. A tissue culture of regenerable cells from the lettuce plant ofparagraph 1.

9. The tissue culture of paragraph 8, wherein said cells or protoplastsof the tissue culture which are derived from a tissue selected from thegroup consisting of leaves, pollen, embryos, cotyledon, hypocotyls,meristematic cells, roots, root tips, anthers, flowers, seeds and stems.

10. A method for producing a progeny plant of a lettuce plant ofparagraph 1, comprising crossing the plant of paragraph 1 with itself orwith another lettuce plant, harvesting the resultant seed, and growingsaid seed.

11. A progeny plant produced by the method of paragraph 10, wherein saidprogeny exhibits a combination of traits including resistance againstdowny mildew strains Bl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae),currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, and lettucemosaic virus (LMV), as well as having intensely red, deeply incised,three-dimensional leaves, and a medium to small plant size.

12. A progeny plant produced by the method of paragraph 10, wherein saidprogeny plant has all the morphological and physiologicalcharacteristics of the lettuce plant designated 79-65 RZ, representativeseed of which having been deposited under NCIMB Accession No. 42179,wherein said plant comprises at least the following combination oftraits including resistance against downy mildew strains Bl:1 to Bl:31,CA-I to CA-VIII (Bremia lactucae), currant-lettuce aphid (Nasonoviaribisnigri) biotype Nr:0, and lettuce mosaic virus (LMV), as well ashaving intensely red, deeply incised, three-dimensional leaves, and amedium to small plant size.

13. A progeny plant produced by the method of paragraph 10, wherein saidprogeny plant, representative seed of which having been deposited underNCIMB Accession 42179, is modified in one or more other characteristics.

14. The progeny of paragraph 13, wherein the modification is effected bymutagenesis.

15. The progeny of paragraph 13, wherein the modification is effected bytransformation with a transgene.

16. A method of producing a hybrid lettuce seed comprising crossing afirst parent lettuce plant with a second parent lettuce plant andharvesting the resultant hybrid lettuce seed, wherein said first parentlettuce plant or said second parent lettuce plant is the lettuce plantof paragraph 1.

17. A hybrid lettuce plant produced by the method of paragraph 16.

18. A method of producing a lettuce cultivar containing a combination oftraits including resistance against downy mildew strains Bl:1 to Bl:31,CA-I to CA-VIII (Bremia lactucae), currant-lettuce aphid (Nasonoviaribisnigri) biotype Nr:0, and lettuce mosaic virus (LMV), as well ashaving intensely red, deeply incised, three-dimensional leaves, and amedium to small plant size, comprising: crossing a mother lettuce plantwith a father lettuce plant to produce a hybrid seed; growing saidhybrid seed to produce a hybrid plant; selfing said hybrid plant toproduce F2 progeny seed; growing said F2 progeny seed to produceF2-plants; selecting said F2-plants for exhibiting resistance againstdowny mildew strains Bl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae),currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, and lettucemosaic virus (LMV), as well as having intensely red, deeply incised,three-dimensional leaves, and a medium to small plant size, and, selfingsaid selected F2-plants to produce F3 progeny seed; growing said F3progeny seed to produce F3-plants; selecting F3-plants for exhibitingresistance against downy mildew strains Bl:1 to Bl:31, CA-I to CA-VIII(Bremia lactucae), currant-lettuce aphid (Nasonovia ribisnigri) biotypeNr:0, and lettuce mosaic virus (LMV), as well as having intensely red,deeply incised, three-dimensional leaves, and a medium to small plantsize, optionally followed by more selfing and selection steps.

19. A lettuce cultivar produced by the method of paragraph 18.

20. A method for producing lettuce leaves as a fresh vegetablecomprising packaging leaves of a plant of paragraph 1.

21. A method for producing lettuce leaves as a processed food comprisingprocessing leaves of a plant of paragraph 1.

22. One or more lettuce plants of paragraph 1, in a container, forharvest of leaves.

23. A lettuce plant having morphological and/or physiologicalcharacteristics of a lettuce plant, representative seed of which havingbeen deposited under NCIMB Accession No. 42179.

24. A method of introducing a desired trait into a plant of lettucevariety 79-65 RZ comprising: (a) crossing a plant of lettuce variety79-65 RZ, representative seed of which having been deposited under NCIMBAccession No. NCIMB 42179, with a second lettuce plant that comprisesthe desired trait to produce F1 progeny; (b) selecting an F1 progenythat comprises the desired trait; (c) crossing the selected F1 progenywith a plant of lettuce variety 79-65 RZ, to produce backcross progenyand (d) selecting backcross progeny comprising the desired trait and thephysiological and morphological characteristic of a plant of lettucevariety 79-65 RZ, when grown in the same environmental conditions.

25. The method of paragraph 24 further comprising (e) repeating steps(c) and (d) one or more times in succession to produce selected fourthor higher backcross progeny that comprise the desired trait and all ofthe physiological and morphological characteristics of a plant oflettuce variety 79-65 RZ, when grown in the same environmentalconditions.

26. A lettuce plant produced by the method of paragraph 24 or 25.

27. A method for producing a seed of a 79-65 RZ-derived lettuce plantcomprising (a) crossing a plant of lettuce variety 79-65 RZ,representative seed of which having been deposited under NCIMB AccessionNo. NCIMB 42179, with a second lettuce plant, and (b) whereby seed of a79-65 RZ-derived lettuce plant form.

28. The method of paragraph 27 further comprising (c) crossing a plantgrown from 79-65 RZ-derived lettuce seed with itself or with a secondlettuce plant to yield additional 79-65 RZ-derived lettuce seed, (d)growing the additional 79-65 RZ-derived lettuce seed of step (c) toyield additional 79-65 RZ-derived lettuce plants, and (e) repeating thecrossing and growing of steps (c) and (d) for an additional 3-10generations to generate further 79-65 RZ-derived lettuce plants.

29. The method of paragraph 27 or 28 further comprising selecting atsteps b), d), and e), a 79-65 RZ-derived lettuce plant, exhibiting acombination of traits including resistance against downy mildew strainsBl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae), currant-lettuce aphid(Nasonovia ribisnigri) biotype Nr:0, and lettuce mosaic virus (LMV), aswell as having intensely red, deeply incised, three-dimensional leaves,and a medium to small plant size.

30. A seed produced by the method of paragraphs 27, 28, or 29.

31. A method of determining the genotype of a plant of lettuce variety79-65 RZ, representative seed of which has been deposited under NCIMBAccession No. NCIMB 42179, or a first generation progeny thereof,comprising obtaining a sample of nucleic acids from said plant andcomparing said nucleic acids to a sample of nucleic acids obtained froma reference plant, and detecting a plurality of polymorphisms betweenthe two nucleic acid samples, wherein the plurality of polymorphisms areindicative of lettuce variety 79-65 RZ and/or give rise to theexpression of any one or more, or all, of the morphological andphysiological characteristics of lettuce variety 79-65 RZ of paragraph1.

32. The method of paragraph 31 additionally comprising the step ofstoring the results of detecting the plurality of polymorphisms on acomputer readable medium, or transmitting the results of detecting theplurality of polymorphisms.

33. The computer readable medium of paragraph 32.

Having thus described in detail preferred embodiments of the presentinvention, it is to be understood that the invention is not to belimited to particular details set forth in the above description as manyapparent variations thereof are possible without departing from thespirit or scope of the present invention.

What is claimed is:
 1. A lettuce plant designated 79-65 RZ,representative seed of which having been deposited under NCIMB AccessionNo. 42179, wherein said plant comprises at least the followingcombination of traits including resistance against downy mildew strainsBl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae), currant-lettuce aphid(Nasonovia ribisnigri) biotype Nr:0, and lettuce mosaic virus (LMV), aswell as having intensely red, deeply incised, three-dimensional leaves,and a medium to small plant size.
 2. A seed of the plant of claim
 1. 3.A part of the plant of claim 1, wherein said part of the plant issuitable for sexual reproduction.
 4. The part of the plant as claimed inclaim 3, wherein said part comprises a microspore, pollen, ovary, ovule,embryo sac or egg cell.
 5. A part of the plant of claim 1, wherein saidpart of the plant is suitable for vegetative reproduction.
 6. The partas claimed in claim 5, wherein said part comprises a cutting, root,stem, cell or protoplast.
 7. A tissue culture of regenerable cells orprotoplasts from the lettuce plant of claim
 1. 8. The tissue culture asclaimed in claim 7, wherein said cells or protoplasts of the tissueculture are derived from a tissue comprising a leaf, pollen, embryo,cotyledon, hypocotyl, meristematic cell, root, root tip, anther, flower,seed or stem.
 9. A method for producing a progeny plant of a lettuceplant of claim 1, comprising crossing the plant of claim 1 with itselfor with another lettuce plant, harvesting the resultant seed, andgrowing said seed.
 10. A progeny plant produced by the method of claim9, wherein said progeny exhibits a combination of traits includingresistance against downy mildew strains Bl:1 to Bl:31, CA-I to CA-VIII(Bremia lactucae), currant-lettuce aphid (Nasonovia ribisnigri) biotypeNr:0, and lettuce mosaic virus (LMV), as well as having intensely red,deeply incised, three-dimensional leaves, and a medium to small plantsize.
 11. The progeny plant produced by the method of claim 9, whereinsaid progeny plant has all the morphological and physiologicalcharacteristics of the lettuce plant designated 79-65 RZ, representativeseed of which having been deposited under NCIMB Accession No.
 42179. 12.The progeny plant produced by the method of claim 9, wherein saidprogeny plant, representative seed of which having been deposited underNCIMB Accession No. 42179, is further modified in one or more othercharacteristics.
 13. The progeny plant as claimed in claim 12, whereinthe modification is effected by mutagenesis.
 14. The progeny plant asclaimed in claim 12, wherein the modification is effected bytransformation with a transgene.
 15. A method of producing a hybridlettuce seed comprising crossing a first parent lettuce plant with asecond parent lettuce plant and harvesting the resultant hybrid lettuceseed, wherein said first parent lettuce plant or said second parentlettuce plant is the lettuce plant of claim
 1. 16. A hybrid lettuce seedproduced by the method of claim
 15. 17. A hybrid lettuce plant grownfrom the seed as claimed in claim
 16. 18. A method for producing lettuceleaves as a fresh vegetable comprising packaging leaves of a plant ofclaim
 1. 19. A method for producing lettuce leaves as a processed foodcomprising processing leaves of a plant of claim
 1. 20. A containercomprising one or more lettuce plants of claim 1 for harvest of leaves.21. A method of determining the genotype of a plant of lettuce variety79-65 RZ, representative seed of which has been deposited under NCIMBAccession No. 42179, or a first generation progeny thereof, comprisingobtaining a sample of nucleic acids from said plant and comparing saidnucleic acids to a sample of nucleic acids obtained from a referenceplant, and detecting a plurality of polymorphisms between the twonucleic acid samples, wherein the plurality of polymorphisms areindicative of lettuce variety 79-65 RZ and/or give rise to theexpression of any one or more, or all, of the morphological andphysiological characteristics of lettuce variety 79-65 RZ as claimed inclaim
 1. 22. The method of claim 21 additionally comprising the step ofstoring the results of detecting the plurality of polymorphisms on acomputer readable medium, or transmitting the results of detecting theplurality of polymorphisms.
 23. The plant of claim 1, which is grownfrom seed having been deposited under NCIMB Accession No.
 42179. 24. Amethod of producing a lettuce cultivar containing a combination oftraits including resistance against downy mildew strains Bl:1 to Bl:31,CA-I to CA-VIII (Bremia lactucae), currant-lettuce aphid (Nasonoviaribisnigri) biotype Nr:0, and lettuce mosaic virus (LMV), as well ashaving intensely red, deeply incised, three-dimensional leaves, and amedium to small plant size, representative seed of which having beendeposited under NCIMB Accession No. 42179, comprising: crossing a motherlettuce plant with a father lettuce plant to produce a hybrid seed;growing said hybrid seed to produce a hybrid plant; selfing said hybridplant to produce F2 progeny seed; growing said F2 progeny seed toproduce F2-plants; selecting said F2-plants for exhibiting resistanceagainst downy mildew strains Bl:1 to Bl:31, CA-I to CA-VIII (Bremialactucae), currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0,and lettuce mosaic virus (LMV), as well as having intensely red, deeplyincised, three-dimensional leaves, and a medium to small plant size,and, selfing said selected F2-plants to produce F3 progeny seed; growingsaid F3 progeny seed to produce F3-plants; selecting F3-plants forexhibiting resistance against downy mildew strains Bl:1 to Bl:31, CA-Ito CA-VIII (Bremia lactucae), currant-lettuce aphid (Nasonoviaribisnigri) biotype Nr:0, and lettuce mosaic virus (LMV), as well ashaving intensely red, deeply incised, three-dimensional leaves, and amedium to small plant size, representative seed of which having beendeposited under NCIMB Accession No. 42179, optionally followed by moreselfing and selection steps.
 25. A lettuce cultivar produced by themethod of claim
 24. 26. A lettuce plant having resistance against downymildew strains Bl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae),currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, and lettucemosaic virus (LMV), as well as having intensely red, deeply incised,three-dimensional leaves, and a medium to small plant size and othermorphological and/or physiological characteristics of a lettuce plant,representative seed of which having been deposited under NCIMB AccessionNo.
 42179. 27. A method of introducing a desired trait into a plant oflettuce variety 79-65 RZ comprising: (a) crossing a plant of lettucevariety 79-65 RZ, representative seed of which having been depositedunder NCIMB Accession No. 42179, with a second lettuce plant thatcomprises the desired trait to produce F1 progeny; (b) selecting an F1progeny that comprises the desired trait; (c) crossing the selected F1progeny with a plant of lettuce variety 79-65 RZ, to produce backcrossprogeny and (d) selecting backcross progeny comprising the desired traitand physiological and morphological characteristics of a plant oflettuce variety 79-65 RZ, when grown in the same environmentalconditions.
 28. The method of claim 27 further comprising (e) repeatingsteps (c) and (d) one or more times in succession to produce selectedfourth or higher backcross progeny that comprise the desired trait andall of the physiological and morphological characteristics of a plant oflettuce variety 79-65 RZ, when grown in the same environmentalconditions.
 29. A lettuce plant produced by the method of claim
 27. 30.A method for producing a seed of a 79-65 RZ-derived lettuce plantcomprising (a) crossing a plant of lettuce variety 79-65 RZ,representative seed of which having been deposited under NCIMB AccessionNo. 42179, with a second lettuce plant, and (b) whereby seed of a 79-65RZ-derived lettuce plant forms.
 31. The method of claim 30 furthercomprising (c) crossing a plant grown from 79-65 RZ-derived lettuce seedwith itself or with a second lettuce plant to yield additional 79-65RZ-derived lettuce seed, (d) growing the additional 79-65 RZ-derivedlettuce seed of step (c) to yield additional 79-65 RZ-derived lettuceplants, and (e) repeating the crossing and growing of steps (c) and (d)for an additional 3-10 generations to generate further 79-65 RZ-derivedlettuce plants.
 32. The method of claim 31 further comprising selectingat steps b), d), and e), a 79-65 RZ-derived lettuce plant, exhibiting acombination of traits including resistance against downy mildew strainsBl:1 to Bl:31, CA-I to CA-VIII (Bremia lactucae), currant-lettuce aphid(Nasonovia ribisnigri) biotype Nr:0, and lettuce mosaic virus (LMV), aswell as having intensely red, deeply incised, three-dimensional leaves,and a medium to small plant size.
 33. A seed produced by the method ofclaim 30.